Hot-off-the-presses publications from the lab. Click on the titles below for a brief descriptor of the project. Alternatively, since not all Mehle Lab pubs are highlighted below, click here for a PubMed listing of all of our publications.
Retasking of canonical antiviral factors into proviral effectors
King and Mehle. (2022)
When is antiviral not antiviral? This review highlights how it is all about context.
Alternative splicing liberates a cryptic cytoplasmic isoform of mitochondrial MECR that antagonizes influenza virus
Baker, et al. (2021)
He we show how an enzyme involved in mitochondrial fatty acid synthesis has a minor isoform that moonlights as an antiviral protein.
Influenza A virus undergoes compartmentalized replication in vivo dominated by stochastic bottlenecks
Amato, et al. (2022)
Why does influenza virus appear to evolve so slowly within an individual? Our data suggest replication islands and genetic bottlenecks restrain evolution.
Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs
Tran*, Ledwith*, et al. (2020)
A mystery to solve when an anti-viral protein is not an anti-viral protein.
The later stages of viral infection: An undiscovered country of host dependency factors
King and Mehle (2020)
What we know, and what we don’t know, about cellular co-factors during infection.
Post-Translation Regulation of Influenza Virus Replication
Dawson, et al. (2020)
Here we review how post-translational modifications afford dynamic and localized control of proteins during viral infection.
Phosphorylation controls RNA binding and transcription by the influenza virus polymerase
Dawson, et al. (2020)
Mechanistic dissection of phosphorylation events on the viral polymerase.
EPS8 Facilitates Uncoating of Influenza A Virus
Larson, Tran, et al. (2019)
We laugh in the face of experimental variability and exploit this to find new host factors regulating flu.
Experimental Approaches to Identify Host Factors Important for Influenza Virus
Schaack and Mehle (2019)
Everything you ever wanted to know about experimental techniques used to find viral host factors.
The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity
Ristow, et al. (2019)
A collaboration that brings the Mehle lab its first paper on bacterial pathogensis.
ANP32B, or not to be, that is the question for influenza virus
Baker and Mehle (2019)
Our synthesis of recent ANP32 papers.
Differential splicing of ANP32A in birds alters its ability to stimulate RNA synthesis by restricted influenza polymerase
Baker, et al. (2018)
How differences in ANP32A coding and splicing impact influenza polymerase activity, and possibly evolution in a diverse host range.
Flu’s cues: Exploiting host post-translational modifications to direct the influenza virus replication cycle.
Dawson and Mehle (2018)
Dynamic control of influenza virus by post-translation modification.
Measuring Influenza Virus Infection Using Bioluminescent Reporter Viruses for In Vivo Imaging and In Vitro Replication Assays
Karlsson, et al. (2018)
Using “glowy” flu to measure replication.
Influenza virus recruits host protein kinase C to control assembly and activity of its replication machinery
Mondal, et al. (2017)
Phosphorylation regulates assembly of the viral replication machinery. He we identify the PKC family of kinases, especially PKCδ, as major players in this regulatory scheme.
The Avian Influenza Virus Polymerase Brings ANP32A Home to Roost
Mehle A (2016)
Avian influenza virus polymerases function poorly in mammals, restricting transmission across species and the emergence of pandemic outbreaks. We discuss new work from the Barclay lab implicating ANP32A as a crucial cellular protein that dictates species-specific polymerase activity.
Influenza: Current Research – Host Factors Regulating the Influenza Virus Replication Machinery
Kirui J, Tran V, Mehle A (2016)
Our book chapter describing host factors that control the influenza polymerase and replication machinery.
Ubiquitination up-regulates influenza polymerase function
Kirui J, Mondal A, Mehle A. (2016)
Ubiquitination and the ubiquitin-proteasome pathway play key roles during multiple stages of influenza virus infection. We now show that these processes modulate viral polymerase activity independent of protein degradation.
Fiat Luc: Bioluminescence Imaging Reveals In Vivo Viral Replication Dynamics
Mehle A (2015)
Our “Pearl” discussing the use of bioluminescence imaging for studying replication dynamics in vivo
Phosphorylation at the homotypic interface regulates nucleoprotein oligomerization and assembly of the influenza virus replication machinery
Mondal A, Potts GK, Dawson AR, Coon JJ, Mehle A (2015)
How does influenza virus regulate assembly of its replication machinery? Here we show that phosphorylation of NP is critical for proper formation of the replication machinery and new virions.
Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
Tran V, Poole DS, Jeffery JJ, Sheahan TP, Creech D, Yevtodiyenko A, Peat AJ, Francis KP, You S, Mehle A (2015)
Developing and applying a diverse collection of reporter viruses to measure virus replication and inflammation in live animals.
Visualizing real-time influenza virus infection, transmission and protection in ferrets
Karlsson EA, Meliopoulos VA, Savage C, Livingston B, Mehle A, Schultz-Cherry S (2015)
In collaboration with the Schultz-Cherry lab, we use bioluminescence imaging to measure replication and transmission between ferrets in real time.
Influenza A virus polymerase is a site for adaptive changes during experimental evolution in bat cells
Poole DS, Yú S, Caì Y, Dinis JM, Müller MA, Jordan I, Friedrich TC, Kuhn JH, Mehle A (2014)
Bats appear to be a reservoir for a large number of viruses that spill over into humans. Following on the recent identification of a unique lineage of influenza virus in bats, we asked what it takes for human influenza viruses to adapt and replicate in bat cells.
Unusual influenza A viruses in bats
Mehle A (2014)
A brief review of the ancient lineage of influenza viruses recently found circulating in wild bat populations in Central and South American. We discuss the unique features of these viruses, their place in the evolution of Orthomyxoviridae and their potential (or not) to cause disease in humans.
Conserved features of the PB2 627 domain impact influenza virus polymerase function and replication
Kirui J, Bucci MD, Poole DS, Mehle A (2014)
The 627 domain of the viral polymerase is a major determinant of host range. Here we identify key structural and biochemical properties required for general activity and species-specific functionality.
Highly sensitive real-time in vivo imaging of an influenza reporter virus reveals dynamics of replication and spread
Tran V, Moser LA, Poole DS, Mehle A (2013)
Stemming from our love-hate relationship with plaque assays, we developed reporter viruses that faithfully recapitulate influenza virus replication and used these to perform longitudinal measures during infections in mice.