Hot-off-the-presses publications from the lab. Click on the titles below for a brief descriptor of the project. Alternatively, since not all Mehle Lab pubs are highlighted below, click here for a PubMed listing of all of our publications.
Mondal, et al. (2017)
Phosphorylation regulates assembly of the viral replication machinery. He we identify the PKC family of kinases, especially PKCδ, as major players in this regulatory scheme.
Baker, et al. (2018)
How differences in ANP32A coding and splicing impact influenza polymerase activity, and possibly evolution in a diverse host range.
Kirui J, Mondal A, Mehle A. (2016)
Ubiquitination and the ubiquitin-proteasome pathway play key roles during multiple stages of influenza virus infection. We now show that these processes modulate viral polymerase activity independent of protein degradation.
Kirui J, Tran V, Mehle A (2016)
Our book chapter describing host factors that control the influenza polymerase and replication machinery.
Mehle A (2016)
Avian influenza virus polymerases function poorly in mammals, restricting transmission across species and the emergence of pandemic outbreaks. We discuss new work from the Barclay lab implicating ANP32A as a crucial cellular protein that dictates species-specific polymerase activity.
Mehle A (2015)
Our “Pearl” discussing the use of bioluminescence imaging for studying replication dynamics in vivo
Tran V, Poole DS, Jeffery JJ, Sheahan TP, Creech D, Yevtodiyenko A, Peat AJ, Francis KP, You S, Mehle A (2015)
Developing and applying a diverse collection of reporter viruses to measure virus replication and inflammation in live animals.
Mondal A, Potts GK, Dawson AR, Coon JJ, Mehle A (2015)
How does influenza virus regulate assembly of its replication machinery? Here we show that phosphorylation of NP is critical for proper formation of the replication machinery and new virions.
Karlsson EA, Meliopoulos VA, Savage C, Livingston B, Mehle A, Schultz-Cherry S (2015)
In collaboration with the Schultz-Cherry lab, we use bioluminescence imaging to measure replication and transmission between ferrets in real time.
Poole DS, Yú S, Caì Y, Dinis JM, Müller MA, Jordan I, Friedrich TC, Kuhn JH, Mehle A (2014)
Bats appear to be a reservoir for a large number of viruses that spill over into humans. Following on the recent identification of a unique lineage of influenza virus in bats, we asked what it takes for human influenza viruses to adapt and replicate in bat cells.
Mehle A (2014)
A brief review of the ancient lineage of influenza viruses recently found circulating in wild bat populations in Central and South American. We discuss the unique features of these viruses, their place in the evolution of Orthomyxoviridae and their potential (or not) to cause disease in humans.
Kirui J, Bucci MD, Poole DS, Mehle A (2014)
The 627 domain of the viral polymerase is a major determinant of host range. Here we identify key structural and biochemical properties required for general activity and species-specific functionality.
Tran V, Moser LA, Poole DS, Mehle A (2013)
Stemming from our love-hate relationship with plaque assays, we developed reporter viruses that faithfully recapitulate influenza virus replication and used these to perform longitudinal measures during infections in mice.