King, Cason R, Dodge, Mackenzie J., MacNeil, Katelyn M., Tessier Tanner M., Mymryk, Joe S., Mehle, Andrew, 2024. Expanding the adenovirus toolbox: reporter viruses for studying the dynamics of human adenovirus replication. Journal of Virology 0, e00207-24. https://doi.org/10.1128/jvi.00207-24

To streamline standard virological assays, we developed a suite of nine fluorescent or bioluminescent replication competent human species C5 adenovirus reporter viruses that mimic their parental wild-type counterpart. These reporter viruses provide a rapid and quantitative readout of various aspects of viral infection and replication based on EGFP, mCherry, or NanoLuc measurement. Moreover, they permit real-time non-invasive measures of viral load, replication dynamics, and infection kinetics over the entire course of infection, allowing measurements that were not previously possible. This suite of replication competent reporter viruses increases the ease, speed, and adaptability of standard assays and has the potential to accelerate multiple areas of human adenovirus research.